Antibody Production and Purification
Sigma Aldrich Fine Chemicals Biosciences Freund’s Adjuvant, Complete, MFCD00131105, 10 mL
Synonym: FCA. Cell suspension. Each mL contains 1 mg of Mycobacterium tuberculosis (H37Ra, ATCC 25177), heat killed and dried, 0.85 mL paraffin oil and 0.15 mL mannide monooleate. Antigens (preferably in saline) are typically mixed with an equal volume of the adjuvant to form an emulsion. Freund′s Adjuvants may be used to produce water-in-oil emulsions of immunogens. Antigens in water-in-oil emulsions stimulate high and long-lasting antibody responses which can be attributed to the slow release of antigen.
Invivogen Adju-Phos adjuvant, 250 mL
Adju-Phos adjuvant is an aluminium phosphate wet gel suspension. It induces a Th2 response by improving the attraction and uptake of antigen by antigen-presenting cells (APCs). It can also activate innate immunity pathways triggered by pattern recognition receptors (PRRs).
Ethos Biosciences hemoglobin HbA0 standard blood 1/EA
Hemoglobin A0 is prepared from lysed human blood cells by sequential ion-exchange It is a mixture of non-glycated and glycated hemoglobin A0 fractions but is essentially free of HbA1a HbA1b HbA1c and HbF The protein is validated by HPIEC methods and is suitable as a primary standard in chromatographic or immunologic analyses The reported concentration is determined by Drabkins assay The material is supplied in liquid form in dilute potassium cyanide-phosphate buffer The concentration varies with manufacturing lot but the protein may be used directly by dilution in buffer in most experimental methods It is shipped frozen at -20oCShort term storage should be at 4oC Long term storage should be at -60oC Repeated cycles of freezing and thawing should be avoided